Synthesis, In-vitro Antioxidant, Anti-diabetic Evaluation and Docking Studies of Newly Synthesized Benzoxazole Derivatives

In the present study, a set of different benzoxazole derivatives has been synthesized from ethyl acetoacetate, ethoxymethylene malononitrile, NaNO2, and organic acids. Analytical instruments like proton NMR (1H), carbon (13C), infrared spectroscopy (IR), LC-MS mass spectrometry were used for structural characterization. Synthesized molecules evaluated In-vitro antioxidant property (DPPH assay, Total & reducing power method) anti-diabetic (alpha-amylase alpha-glucosidase assay). silico, studies against Human pancreatic alpha-amylase (PDB ID: 3BAW) have carried out to get binding approach ligand towards protein. The results demonstrated that compounds namely 5b, 6b, 3b 4b had potent activity compared with ascorbic acid acarbose. HIGHLIGHTS Anti-oxidant Reducing power) Anti-diabetic assay) activities performed Sulfonamide substitutions are more biological silico docking correlate in vitro small three-dimensional, stable structure its ability form hydrogen bonding show good GRAPHICAL ABSTRACT